Figure 1From: Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancerGeNorm analysis of the candidate reference genes. (A) Average expression stability values of reference genes. Genes are presented in an increasing order of stability from left to right with ACTB being the least stable gene and HPRT1 and PPIA the most stable genes. (B) Determination of optimal number of control genes for normalization. Every bar represents change in normalization accuracy when stepwise adding more reference genes according to ranking in Figure 1. A. To meet the recommended cut-off value of 0.15 two pr three genes would be satisfactory for normalization.Back to article page