Journal of Experimental & Clinical Cancer Research

Table 2 Cycle threshold (Ct) values of candidate reference genes divided in the four tissue groups.

From: Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

Gene symbol	Non-metastatic colon cancer						Metastatic colon cancer
	Tumour			Normal			Tumour			Normal
	Mean	SD	N	Mean	SD	N	Mean	SD	N	Mean	SD	N
18S	8,095	0,546	18	8,440	1,066	18	8,800	1.066	20	9,408	2,035	20
ACTB	20,003	0,765	18	19,949	1,209	18	20,363	1.209	20	20,578	2,673	20
B2M	17,050	0,996	18	17,041	1,002	18	17,217	1.002	20	17,085	1,632	20
GAPDH	18,503	0,722	18	19,502	1,044	18	19,211	1.044	20	20,145	2,541	20
GUSB	23,274	0,375	18	24,081	0,865	18	23,564	0.865	20	24,060	1,981	20
HMBS	25,328	0,736	18	26,577	0,974	18	25,963	0.974	20	27,030	2,436	20
HPRT1	22,795	0,814	18	24,183	0,750	18	23,320	0.750	20	24,264	1,849	20
IPO8	24,575	0,469	18	25,084	0,780	18	25,099	0.780	20	25,529	2,108	20
PGK1	20,322	1,054	18	21,151	1,012	18	20,996	1.011	20	21,573	3,257	20
POLR2A	24,007	0,634	18	24,508	1,061	18	24,933	1.061	20	25,330	2,590	20
PPIA	17,081	0,485	18	18,241	0,906	18	17,506	0.906	20	18,335	1,724	20
RPLP0	19,706	0,637	18	20,647	0,952	18	20,319	0.952	20	21,081	2,002	20
TBP	26,157	0,577	18	26,860	1,035	18	26,649	1.035	20	27,110	2,797	20
TFRC	21,774	0,926	18	23,334	1,030	18	22,679	1.030	20	23,663	2,303	20
UBC	21,285	0,675	18	21,771	1,046	18	21,532	1.046	20	22,044	2,180	20
YWHAZ	23,933	0,723	18	25,041	1,275	18	24,457	1.275	20	25,401	2,174	20

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ISSN: 1756-9966

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