Fig. 5

WTAP directly bound to CDK2 transcript and regulated its mRNA stablility. a Knockdown of WTAP could shorten the half-life of CDK2 transcript. b Ectopic expression of WTAP could lengthen the half-life of CDK2 transcript. c WTAP could bind to CDK2 transcript in vivo in RCC cells. RCC cell lysates were immunoprecipitated with WTAP antibody or control IgG followed by RT-PCR and qRT-PCR. d The luciferase activity for the reporter carrying CDK2 3′-UTR was increased by WTAP overexpression. Caki-1 and ACHN cells with WTAP overexpression and the control were transfected with pGL3 control reporter or pGL3 reporter carrying CDK2 3′-UTR. Data represent the mean ± SD from three independent experiments,*P < 0.05