Fig. 1From: (Arg)9-SH2 superbinder: a novel promising anticancer therapy to melanoma by blocking phosphotyrosine signaling(Arg)9-GST SH2 TrM can effectively bind with pY proteins in B16F10 cells. The whole cell lysates of B16F10 cells with or without EGF treatment were incubated with (Arg)9-GST, (Arg)9-GST SH2 Wt or (Arg)9-GST SH2 TrM for 12 h at 4 °C. The pY-containing proteins were enriched, pulled down and then examined by western blot using anti-pY antibody. Data shown are representative of three independent experimentsBack to article page