Fig. 2

(Arg)₉-GST SH2 TrM could efficiently enter into B16F10 cells. Effects of (Arg)9-GST SH2 TrM translocating into B16F10 cells at different concentrations (0.5, 0.75,1 and 2 μM) (a) and various time (0.5 h,1 h,2 h and 4 h) (b). c Distinct penetration ability of GST, GST SH2 Wt, GST SH2 TrM, (Arg)₉-GST, (Arg)₉-GST SH2 Wt and (Arg)₉-GST SH2 TrM proteins. Cells were stained with anti-GST antibody followed by goat-anti-rabbit FITC secondary antibody. Actin was stained with Rhodamine-phallodin (Red) and nucleus with DAPI (Blue). Scale bar: 20 μm. All images shown are representative of at least three independent experiments