Fig. 3

RNA-seq analyses of the effect of VB1 on the gene expression profile. a A375 cells were treated with 10 μM VB1 for 48 h. RNA-seq was performed as described in the Methods, and differential expression genes were analyzed using DESeq2. b The KEGG pathway was used to analyze the pathways related to the differential expression genes. The top 20 positively enriched pathways are shown in the bubble chart. The x-axis is the enrichment score, and the y-axis is the enriched pathways. c & d RNA was extracted from A375 (c) and Sk-Mel-28 (d) treated with VB1 as indicated, and RT-Q-PCR was then performed as described in the Methods. The data from multiple experiments are expressed as the mean (n = 4) ± S.D. Significant differences were evaluated using one-way ANOVA, and an asterisk (*) indicates a significant difference (p < 0.05)