Fig. 5

CLDN7 overexpression significantly decreased cell migratory and invasive ability in vitro and in vivo. (A) Wound healing assay. a. Left panel showed the representative images of wound of Caki-1 CLDN7 and Control cells at 0 h and 12 h. Scale bar, 100 μm. Relative cell migratory distance of Caki-1 CLDN7 and Control were statistically analyzed (right panel). b. Representative images of wound of A498 CLDN7 and Control cells at 0 h and 36 h. Scale bar, 100 μm (Left panel). Relative cell migratory distance of A498 CLDN7 and Control were statistically analyzed (right panel). (B) Transwell migrantion and invasion assay. a. Representative images of transwell migration assay of Caki-1 and A498 CLDN7 and Control cells for 48 h. Scale bar, 100 μm (Top). Statistically analysis of migratory cells from Caki-1 and A498 CLDN7 and Control cells (bottom). b. Representative images of transwell invasion assay of Caki-1 and A498 CLDN7 and Control cells for 72 h. Scale bar, 100 μm (Top). Statistically analysis of invasive cells from Caki-1 and A498 CLDN7 and Control cells (bottom). (C) Mouse metastasis model. a. Representative images of metastatic lesions derived from Caki-1 Control and CLDN7 cells 20 days after transplanation. It is detected by in vivo imaging system-Xenogen IVIS. Statistical analysis of tumor luminescence (photons/ sec) between Caki-1 Control-Luc and Caki-1 CLDN7-Luc group (bottom panel). b. Representative images of liver (top) and lung (bottom) with metastatic lesions derived from Caki-1 Control-Luc and CLDN7-Luc cells (left panel). Right panel showed the statistical analysis of metastatic nodules in liver and lung per mouse. c. Representative HE staining of liver (top) and lung (bottom) with metastatic lesions derived from Caki-1 Control-Luc and CLDN7-Luc cells. Scale bar, 100 μm. *p < 0.05, **p < 0.01, Student’s Test