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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: TNF-alpha and metalloproteases as key players in melanoma cells aggressiveness

Fig. 1

Characterization of melanoma cell aggressiveness: all the cell lines were analysed simultaneously using the same experimental procedures. Evaluations and quantifications were assessed by two different operators in blind. a Growth rate after 24 and 48 h of serum starvation. After serum deprivation, cells were incubated for 24 and 48 h and then they are harvested and counted. Cell counts for each cell line was: 61729 for SK-MEL-110, 57,250 for Mel 397, 46,171 for SK-MEL-30, 57,000 for Preyer, 61,333 for A375M, 60,212 for A375, 61,143 for SK-MEL-28, 60,600 for Mewo, 60,500 for Mel 501, 60,500 for Me 665. The data represent the mean ± SD of three experiments carried out in triplicate (statistical significance versus control: **P < 0.001; § P < 0.0001). b Migration ability of melanoma cell lines. The scratch test on confluent cells were performed for 24 h. c Invasion analysis of melanoma cells for 24 h. The invasion capability is expressed as number of cells per mm2 of filter. d Aggressiveness index (MAGS index calculated as reported in Methods), to cluster melanoma cell lines accordingly to their malignancy: a combination of growth, invasion and migration rates was used to get such aggressiveness index

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