Fig. 6

Overexpression of SOX9 restores lung metastasis in SOX10 KD melanoma cells. a A375M cells treated with the indicated constructs were injected via the tail vein into NOD/SCID mice (n = 5 per treatment) and the bioluminescence images were taken 2 weeks after injection. H&E staining was used to detect for the presence of tumor tissues in lungs of NOD/SCID mice. Scale bars: 200 μM. b Gross pictures of lungs from xenografted NOD/SCID mice. The red arrowheads indicate the macroscopic lesions. Scale bar: 1.0 cm. c Bioluminescence intensity was measured and plotted. d Quantification of the number of nodules formed on the lung surface of NOD/SCID mice injected with the indicated constructs. e Immunofluorescence detection for SOX10, SOX9, and NEDD9 on the cross-section of lung nodules derived from A375M cells treated with scramble control, SOX10 KD + SOX9 OE and SOX10 KD + NEDD9 OE. Magnification of the boxed regions with an overlapping expression of SOX9 and NEDD9. Scale bar: 100 μM. Error bars represent mean ± SD of three independent experiments. *p < 0.05, p*** < 0.001