Table 1 Candidate reference genes included in the TaqMan Endogenous Control Assay.
Gene name | Gene symbol | Assay ID | Size (bp) | Function |
|---|---|---|---|---|
18S Ribosomal RNA | 18S | Hs99999901_s1 | 187 | Part of a ribosomal subunit |
Phosphoglycerate kinase-1 | PGK1 | Hs99999906_m1 | 75 | Key enzyme in glycolysis |
Î’-Actin | ACTB | Hs99999903_m1 | 171 | Cytoskeletal structural protein |
Polymerase (RNA) II polypeptide A | POLR2A | Hs00172187_m1 | 61 | Catalyzes the RNA synthesis from DNA |
Beta-2-microglobulin | B2M | Hs99999907_m1 | 75 | Beta-chain of major histocompatibility complex class I molecules |
Peptidyl-prolyl isomerase/cyclophilin A | PPIA | Hs99999904_m1 | 98 | Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, accelerating folding |
Glyceraldehyd-3-phosphate dehydrogenase | GAPDH | Hs99999905_m1 | 122 | Dehydrogenase, oxidoreductase in glycolysis and gluconeogenesis |
Acidic ribosomal phosphoprotein P0 | RPLP0 | Hs99999902_m1 | 105 | Ribosome biogenesis and assembly |
Î’-Glucuronidase | GUSB | Hs99999908_m1 | 81 | Glycoprotein, degradation of dermatan and keratin sulfates |
Transcription factor IID, TATA box binding protein | TBP | Hs99999910_m1 | 127 | TATA binding protein, general RNA polymerase II transcription factor |
Hydromethylbilane synthase | HMBS | Hs00609297_m1 | 64 | Heme synthesis, porphyrin metabolism |
Transferrin receptor (p90, CD71) | TFRC | Hs99999911_m1 | 105 | Cellular uptake of iron |
Hypoxanthine-phosphoribosyl-transferase 1 | HPRT1 | Hs99999909_m1 | 100 | Glycosyltransferase, purine synthesis in salvage pathway |
Ubiquitin C | UBC | Hs00824723_m1 | 71 | Protein degradation |
Importin 8 | IPO8 | Hs00183533_m1 | 71 | Function in nuclear protein import |
Tyrosine 3 monooxygenase activation protein, zeta polypeptide | YWHAZ | Hs00237047_m1 | 70 | Signal transduction by binding to phosphorylated serine residues on a variety of signalling molecules |