Figure 5From: Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cellsImmunohistochemical localization of NKG2D in cervical cancer cell lines. Adherent cells were preincubated with 10 ng of MICB for 72 h and then incubated with an anti-NKG2D primary antibody followed by an HRP-conjugated secondary antibody, and the samples were developed with diaminobenzidine and counterstained with methylene blue. Negative control (A), isotype control (B) and NKG2D staining (C) of CALO (left panels) and INBL (right panels) cells. Note the strong cytoplasmic staining in both cell lines. (Original magnification × 40)Back to article page