Table 1 The primers used in the PCR reaction and annealing
Gene name | Sequence (5’to3’) | Direction |
|---|---|---|
CDKN1A | AGCGACCTTCCTCATCCACC | Forward |
CDKN1A | AAGACAACTACTCCCAGCCCCATA | Reverse |
CCNE2 | ATCTCCTGGCTAAATCTCTTTCTCC | Forward |
CCNE2 | ACTGGAACTCTAATGAATCAATGGC | Reverse |
CCNA2 | TTTAGCACTCTACACAGTCACGGGA | Forward |
CCNA2 | GGTGAAGGTCCATGAGACAAGGC | Reverse |
CDK6 | AGAGCAAGATAATAAAGGAGATGGG | Forward |
CDK6 | CATGTGAGACTTTGAGTAGACCTGA | Reverse |
MCM6 | GCTGTCGCACTGTAATCCTCC | Forward |
MCM6 | ATTGATCGTGTCTATTCCCTCG | Reverse |
BBC3 | TCTCCTCTCGGTGCTCCTTCACT | Forward |
BBC3 | ACGTTTGGCTCATTTGCTCTTCA | Reverse |
GADD45A | CTCAAGCAGTTACTCCCTACAC | Forward |
GADD45A | CTTCTTCATTTTCACCTCTTTCCA | Reverse |
CDK1 | TAGTCTGGTCTTTCTTTGGCTG | Forward |
CDK1 | GTTCAAAACTGGAATAAAACACCTA | Reverse |