Fig. 1

ARNTL is hypermethylated in nasopharyngeal carcinoma. a ARNTL promoter CpG islands and bisulfite pyrosequencing region. Blue region, CpG islands; Red region, input sequence; TSS, transcription start site; cg15603424, CG site identified in our previous genome-wide methylation analysis; red text, CG sites for bisulfite pyrosequencing; bold red text, the most methylated CG sites in ARNTL. b Bisulfite pyrosequencing analysis of the ARNTL promoter region in 8 pairs of normal and nasopharyngeal carcinoma tissues. c Average methylation levels of ARNTL in 8 pairs of normal and nasopharyngeal carcinoma tissues. d Methylation levels of ARNTL in NP69 and nasopharyngeal carcinoma cell lines. e Correlation between promoter methylation and mRNA expression of ARNTL in the TCGA head and neck cancer dataset. ** indicated P < 0.01