Fig. 6

MiR-26b-5p represses TRIM44 expression by directly targeting its 3′-UTR. a, Two independent databases were searched to computational predict miRNAs that targeting the 3′-UTR of TRIM44. b, qRT-PCR was used to quantify miR-26b-5p expression levels in 20 matched pairs of melanoma and paratumoral tissues. c, Correlation analyses between TRIM44 mRNA and miR-26b-5p were performed using the Spearman correlation coefficient. TRIM44 mRNA negatively correlated with miR-26b-5p expression. d, The putative miR-26b-5p binding sequence in the 3′-UTR of TRIM44. e, 293 T cells were co-transfected with WT or MUT luciferase reporter plasmids and miR-26b-5p mimics or negative control. Luciferase activity was evaluated 48 h post-transfection. f, qRT-PCR and Western blot were used to quantify TRIM44 expression in the indicated cells. Overexpression of miR-26b-5p in A2058 and A375 cells significantly inhibited the expression of TRIM44 a both the mRNA and protein levels. g, A working model depicting the miR-26b-5p-TRIM44-TLR4-AKT-mTOR axis. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001