Fig. 1
From: MicroRNA-486-3p functions as a tumor suppressor in oral cancer by targeting DDR1
DDR1 is up-regulated in oral cancer patients and cell lines a: Heat map of DDR1 expression in 40 OSCC tissue pairs (GSE37991). Red, overexpression; green, downexpression. b: Validation of DDR1 expression by qRT-PCR in another 46 of OSCC tumors (T) compared with their own adjacent normal tissues (N). DDR1 expression levels are expressed as the log2 ratios. All data are represented as mean ± SD; **p < 0.01.c:Immunohistochemical analysis of DDR1 in OSCC tissue array (#OR601a, Biomax), containing 50 cases of OSCC and 10 cases of adjacent normal tissues. Representative samples showing different levels of DDR1 selected from normal tissues (F1, F2 and F4) and tumor tissues (C5, B6 and C2), respectively. The scale bars in the right upper corners of all photos are equivalent to 100 μm. d: Bar charts show the percentage of DDR1 staining score for the oral specimens in the tissue microarray (#OR601a, Biomax). The DDR1 staining intensity was scored as follows: 0, negative; 1, week;2, mediate and 3, strong.e: Western blot analysis of DDR1 expression in normal oral keratinocyte (OKF4/hTERT) and OSCC cell lines (OEC-M1 and TW2.6). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control.f: Kaplan-Meier survival analyses of DDR1 in overall survivalsurvival of patients with oral cancer was determined in our cohort (n = 75). g: Kaplan-Meier survival analyses of DDR1 in overall survival of patients with OSCC cancer was determined in TCGA’s cohort (n = 163)