Fig. 2

Silencing of lncRNA PAXIP1-AS1 inhibits migration, invasion, and angiogenesis of glioma cells. a, RT-qPCR was used to identify the efficiency of overexpressing or silencing lncRNA PAXIP1-AS1 in TJ905 cells. b, Transwell assay to detect the migration ability of TJ905 cells (200 ×). c, Transwell assay to detect the invasive ability of TJ905 cells (200 ×). d, The tube formation assay to detect angiogenesis in HUVECs cocultured with TJ905 cells (100 ×). e, Western blot analysis to determine the expression of E-Cadherin, N-Cadherin, MMP-2 and VEGF-A normalized to GAPDH in TJ905 cells. * p < 0.05 vs. the oe-NC + sh-NC group. The values are measurement data and expressed as mean ± standard deviation. Differences among multiple groups were compared using one-way ANOVA with Tukey’s post hoc test. Cell experiments were repeated three times independently