Fig. 7

KDM5A represses MOB3B and then elicits proliferation, migration and invasion of PCa cells while reducing cell apoptosis via the miR-495/YTHDF2 axis. a, Correlation analysis of KDM5A and MOB3B in PCa tissues. b, The mRNA expression of KDM5A, YTHDF2 and MOB3B in PC3 cells with varied transfection detected by RT-qPCR. c, Western blots of KDM5A, YTHDF2 and MOB3B proteins in cells with varied transfection. d, miR-495 expression in cells following varied transfection detected by RT-qPCR. e, The m6A modification level of MOB3B in cells following varied transfection assessed using Me-RIP. f, PC3 cell proliferation following varied transfection measured by Colony formation assay. g, PC3 cell apoptosis following varied transfection measured by flow cytometry. h, PC3 cell invasion following varied transfection measured by Transwell assay. Scale bar = 50 μm. i, PC3 cell migration following varied transfection measured by Scratch test (Scale bar = 200 μm). * p < 0.05 vs. cells transfected with oe-NC. # p < 0.05 or ^ns p > 0.05 vs. cells transfected with oe-KDM5A + oe-NC. Data in panel a-h were analyzed by one-way ANOVA with Tukey’s post hoc test