Fig. 1

Circ-LRIG3 is upregulated in HCC tissues, cells and plasma. a, b qRT-PCR analysis of circ-LRIG3 expression in adjacent normal tissues (ANT), HCC tissues and cell lines. c The diagrammatic sketch showing the source of circ-LRIG3, and this was confirmed by Sanger sequencing. d The schematic diagram of design of divergent and convergent primers. e PCR analysis using designed divergent and convergent primers in cDNA and gDNA from HCC tissue and SMMC-7721 cells. f, g qRT-PCR analysis of circ-LRIG3 and linear LRIG3 expression in LO2 and SMMC-7721 cells after treatment with actinomycin D or RNase R. (H) The overall and disease-free survival curves of HCC patients with low and high circ-LRIG3 expression. i qRT-PCR analysis of circ-LRIG3 in plasma from HCC and healthy controls. j ROC determining the non-invasive diagnostic value of circ-LRIG3 in HCC based on plasma circ-LRIG3 expression. (K) FISH detecting the subcellular localization of circ-LRIG3 in HCC cells. *P < 0.05, **P < 0.01,***P < 0.001