Fig. 4

Enhanced glycolysis is critically implicated in LNCAROD-induced HCC malignant behaviors. A Heatmap showing the differentially expressed genes following LNCAROD knockdown. B KEGG analysis of the enriched pathways in HCC cells with LNCAROD knockdown. Effects of LNCAROD knockdown on (C) glucose consumption (top), lactate production (middle), and ATP production (bottom) and (D) extracellular acidification rate (ECAR) in SNU-449 and HCC-LM3 cells. Effect of 2-deoxy-D-glucose (2-DG) treatment on the (E) viability, (F) colony formation, (G) migration and invasion, and (H) EMT marker expression of HCC cells. I Effect of LNCAROD knockdown on the mRNA expression of PKM1, PKM2, and PFKL in HCC cells. Data are shown as the mean ± SD. ^*P < 0.05; ^**P < 0.01