Fig. 2From: RNA m6A demethylase FTO-mediated epigenetic up-regulation of LINC00022 promotes tumorigenesis in esophageal squamous cell carcinomaLINC00022 facilitates ESCC proliferation in vitro. (A) The knockdown efficiencies of three specific siRNAs on LINC00022 in KYSE150 and TE1 cells were tested by qRT-PCR, ***p < 0.001. (B) The expression of LINC00022 in KYSE70 cells infected with recombinant over-expressed lentivirus (OE-022) was determined by qRT-PCR, **p < 0.01. (C-E) CCK-8 assay was carried out to evaluate cell viability of KYSE150 and TE1 with LINC00022 knockdown (C, D), and KYSE70 with LINC00022 over-expression (E), *p < 0.05; **p < 0.01. (F-G) Ablation of LINC00022 attenuated the colony formation ability of KYSE150 and TE1 (F), while over-expression of LINC00022 enhanced the colony formation ability of KYSE70 (G), *p < 0.05; **p < 0.01; ***p < 0.001. (H-I) EdU staining assay was conducted to examine cell proliferation of KYSE150 and TE1 with LINC00022 knockdown (H), and KYSE70 with LINC00022 over-expression (I), *p < 0.05; **p < 0.01. Scale bar = 100 μmBack to article page