Fig. 3

ERCC6L accelerates the G₂/M phase transition of BC cells by regulating the p53/p21/CDK1/Cyclin B and Aurora A/PLK/CDC25C/CDK1/Cyclin B pathways. (A) ERCC6L overexpression reduced the proportion of G₂/M phase cells in T47D and MCF-7 cells, while ERCC6L knockdown induced G₂/M phase arrest. (B) Quantification of the flow cytometry cell cycle assay (n = 3). (C) The p53/p21/CDK1/Cyclin B signalling pathway in the G2/M checkpoint was regulated by ERCC6L. (D) Quantification of p53/p21/CDK1/Cyclin B signal pathway-related proteins. (E) The Aurora A/PLK/CDC25C/CDK1/Cyclin B signalling pathway in the G₂/M checkpoint was regulated by ERCC6L. (F) Quantification of Aurora A/PLK/CDC25C/CDK1/Cyclin B signal pathway-related proteins. The results from Western blotting were representative of three independent experiments and then quantified by densitometry using Image J V1.8.0. Data are shown as the means ± SDs. Student’s t test or one-way ANOVA was applied to analyze the statistical significance. ***P < 0.001, **P < 0.01, *P < 0.05