Fig. 6

Circ-RAPGEF5 exerts its carcinogenic effects on ICC cells by promoting SAE1. A CCK-8 cell viability assay for RBE and CCLP1 cells transfected with Si-NC or Si-Circ-RAPGEF5 and vector or SAE1. B EdU labelling assays for RBE and CCLP1 cells transfected with Si-NC or Si-Circ-RAPGEF5 and vector or SAE1. C Colony formation assays for RBE and CCLP1 cells transfected with Si-NC or Si-Circ-RAPGEF5 and vector or SAE1. D Matrigel-coated transwell assays for RBE and CCLP1 cells transfected with Si-NC or Si-Circ-RAPGEF5 and vector or SAE1. All data are presented as the means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001