Fig. 10

Cirbp silencing-induced inhibition of DNA damage repair. A, B Representative pictures of 53BP1 staining (red) A and quantification of the fraction of 53BP1⁺ cells B in shCirbp-expressing NPC cells treated with or without hyperthermia at 42 °C for 30 min. C, D Representative pictures of γ-H2AX staining(red) C and quantification of the fraction of γ-H2AX⁺ D in shCirbp-expressing NPC cells treated with or without hyperthermia at 42 °C for 30 min. E Western blot assay was used to detect Cirbp, p-ATM, p-ATR, p-Chk1, p-Chk2, p-BRCA1, p-p53 and γ-H2AX in indicated cells