Fig. 11

Ectopic expression of Cirbp counteracts the thermosensitivity of NPC cells by promoting DNA damage repair. A, B Representative pictures of 53BP1 staining(red) A and quantification of the fraction of 53BP1⁺ cells B in Cirbp-expressing NPC cells treated with or without hyperthermia at 42 °C for 30 min. C, D Representative pictures of γ-H2AX staining(red) C and quantification of the fraction of γ-H2AX⁺ D in Cirbp-expressing NPC cells treated with or without hyperthermia at 42 °C for 30 min. E Heatmap showing selected differentially expressed genes (see Table S10) involved in DNA damage and repair, and cell cycle in Cirbp-expressing CNE2 and SUNE1 cells. Right column lists the selected gene symbols. F GO and KEGG pathway analysis of up- and down-regulated genes (see Tables S11 and S12) involved in DNA damage and repair, and cell cycle in Cirbp-expressing CNE2 and SUNE1 cells. G Cirbp, p-ATM, p-ATR, p-Chk1, p-Chk2, p-BRCA1, p-p53 and γ-H2AX in indicated cells were determined by Western blot